Product Details FAQ Specification Instructions

In this kit, RNA viruses synthesize cDNA strands using RNA as templates under the action of reverse transcriptase.Qualitative detection of Avian influenza virus H5/H7/H9 subtype by polymerase chain reaction (PCR) combined with Taqman fluorescence probe technique under the action of polymerase.

Sample Type: Animal plasma, serum, swab, tissue, and virus culture can be selected.

High Sensitivity: The detection limit can reach 200copies/ml.

High Specificity: It can effectively distinguish H5/H7/H9 subtypes, and has no cross reaction with H1N1, H3N2, influenza B virus, Newcastle disease virus, infectious bursa of fabricius disease virus, avian infectious bronchitis virus, avian infectious laryngotracheitis virus, chicken Marek's disease virus, mycoplasma gallisepticum, salmonella, and chicken genome DNA.

Wide Applicability: Can be paired with various fluorescent quantitative PCR instruments on the market, especially supporting Bioer Real-Time PCR instruments.

Good Stability: The reagent kit adopts the form of lyophillization. After lyophillization, the active substances (such as enzymes) in the product will be protected at ambient temperature, and can be transported at room temperature, improving and extending the stability of the reagent, greatly reducing the problems of reagent performance degradation or failure caused by temperature changes.

Simple Operation and Short Time Consumption: The reagent kit does not need to be prepared, and after re-dissolution, it only needs to be packaged in one step to add samples for testing, and the test results can be obtained within 1 hour.

The Avian Influenza Virus H5/H7/H9 Subtype Lyophilized Nucleic Acid Detection Kit (Fluorescence PCR) is utilized in veterinary diagnostics, particularly in the detection and surveillance of avian influenza viruses in poultry populations. This kit enables the sensitive and specific detection of H5, H7, and H9 subtypes of avian influenza virus in various sample types, including swabs, tissues, and environmental samples from poultry farms. It plays a crucial role in monitoring and controlling avian influenza outbreaks, facilitating early detection and intervention to prevent the spread of the virus and minimize its impact on poultry production and public health.

10⁴ copies/mL of avian influenza H5N1, H7N9, and H9N2 positive samples were repeat 10 times using the avian influenza virus H5/H7/H9 subtype Lyophilized nucleic acid detection kit (fluorescence PCR method). After statistical analysis, it was found that the co-efficient of variation (CV) of Ct values for each subtype detection result was less than 1%, indicating that the precision of the kit was good and the experimental results were reliable.

1. The unopened kit should be stored at -25℃~8℃ away from light.

2. The kit can be stored for up to 12 months if all components are kept in the manner above. Do not use after the stated expiry date.

3. Don’t open aluminum pouch unless you are ready to use the kit.

4. The lyophilized reagents (reaction mixture) reconstituted with water should be stored at -25℃~-15℃ away from light for up to 1 week. Avoid repeated freeze-thaw (no more than 2 times).

5. The kit can be transported at up to 37℃ for no more than 1 month.