Dengue virus belongs to the Flavivirus genus of the Flaviviridae family. It is a single-stranded positive-sense RNA virus with spherical viral particles approximately 55 nm in diameter. Based on antigenic differences, it is classified into four serotypes: 1, 2, 3, and 4.
Dengue virus is primarily transmitted by vector insects such as Aedes aegypti and Aedes albopictus mosquitoes. It causes dengue fever as well as dengue hemorrhagic fever and dengue shock syndrome, which have high morbidity and mortality rates. Typical clinical manifestations of dengue fever include sudden onset of high fever, headache, severe muscle and joint pain (often described as "breakbone fever"), and in some patients, rash, bleeding tendencies, lymphadenopathy, leukopenia, and thrombocytopenia.
Workflow:
Dengue fever is a mosquito-borne viral infection that causes flu-like symptoms, including high fever, severe headache, pain behind the eyes, joint and muscle pain, rash, and mild bleeding. Nucleic acid testing, due to its rapid speed and high sensitivity, can be widely used for dengue nucleic acid detection. The commonly used sample types for dengue nucleic acid testing are blood and serum. We offer three kits for extracting viral nucleic acids from blood, BSC86, BSC71 and BSC110.
Ordering Information:
Dengue Virus Nucleic Acid Detection Kit (Fluorescent PCR) CE-IVD
This kit uses fluorescent PCR and is compatible with the real-time fluorescence quantitative PCR analyzer (model: FQD-96C, FQD-96A) and the Automated Nucleic Acid Purification and Real Time PCR System (model: FQD-A1600). The kit features rapid detection, high specificity, and high sensitivity. The kit targets highly conserved regions of the dengue virus, with specifically designed primers and probes that bind to the target sequence. Under the action of reverse transcriptase and hot-start Taq DNA polymerase, the specific fragment is amplified. During the amplification process, the fluorescent probe is hydrolyzed, producing fluorescence. The detection system includes dUTP-UDG enzyme contamination prevention measures to fully degrade potential product contamination and avoid false-positive results. Additionally, an internal control is set for a highly conserved region of the human genome. This internal control monitors the entire process of sample collection, transportation, nucleic acid extraction, and PCR amplification, ensuring the validity of the entire process and avoiding false-negative results.
Specification:
Ordering Information:
Dengue IgM/IgG Rapid Test Kit (Colloidal Gold Method)
